During the summer of 2005, plants of potato (Solanum tuberosum L.) showing severe basal rot symptoms were observed in a commercial field near Alessandria (northern Italy). The first symptoms were detected during early July in correspondence with a strong increase of air temperature (as much as 38°C, with an average monthly increase of 10°C) and relative humidity. Infected plants showed dry collar rots and extensive necrosis of cortical tissues. Leaves of infected plants were chlorotic. As the disease progressed, tubers rotted and plants wilted. Infected plants appeared in patches, encompassing 10 to 15% of the cultivated area. In the presence of abundant moisture, a white mycelium occurred on infected tissues. On their surface, infected tubers showed a fan-like mycelial growth. Later, white or light-to-dark brown sclerotia (2 to 4 mm in diameter) developed from mycelium. Clamp connections were present. The diseased tissue was disinfested for 1 min in 1% NaOCl and plated on potato dextrose agar (PDA) amended with 100 mg/liter of streptomycin sulfate. Sclerotium rolfsii was consistently isolated from infected plants. Pathogenicity of one isolate obtained from infected plants was confirmed by inoculating healthy S. tuberosum plants (cv. Hermes) grown in 2:l volume pots (1 plant per pot, seven replicates). Inoculum that consisted of 1 g/pot of wheat kernels infested with mycelium and sclerotia was placed on the soil surface. Seven noninoculated plants served as controls. The inoculation trial was repeated once. Plants were kept at temperatures ranging between 25 and 32°C and watered as needed. Inoculated plants developed symptoms of leaf yellowing within 10 days, soon followed by the appearance of white mycelium and sclerotia and then eventually wilted. Control plants remained symptomless. Sclerotium rolfsii was reisolated from inoculated plants.Possible attacks of Sclerotium rolfsii on S. tuberosum were described as rarely occurring in southern Italy (3). The disease, detected at the moment in very few farms and on cvs. Hermes and Monalisa, was particularly severe on the last variety, causing 5 to 15% yield losses because of premature plant death and rotting of tubers. This disease has been reported in several countries such as India (1), Israel (2), and the United States of America (4).
References: (1) N. S. Bisht. Indian Phytopathol. 35:148, 1982. (2) Y. Elad et al. Soil Biol. Biochem. 16:381, 1984. (3) R. Gigante. Ital. Agric. 87:263, 1946. (4) G. F. Weber. Phytopathology, 33:615, 1943.
