August
2007
, Volume
91
, Number
8
Pages
932
-
941
Authors
M.
Turina
,
Istituto di Virologia Vegetale, CNR, Strada delle Cacce 73, 10135 Torino, Italy
;
M. D.
Ricker
,
Nunhems-USA, Acampo, CA, USA
; and
R.
Lenzi
,
V.
Masenga
, and
M.
Ciuffo
,
Istituto di Virologia Vegetale, CNR, Strada delle Cacce 73, 10135 Torino, Italy
Affiliations
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RelatedArticle
Accepted for publication 21 March 2007.
Abstract
ABSTRACT
We were able to mechanically transmit a small isometric virus from field tomato samples showing severe necrotic symptoms, collected in the Culiacan area of Sinaloa state (Mexico). After gradient purification and three rounds of single-lesion passage on Chenopodium quinoa, the virus was back-inoculated to tomato plants and reproduced the original apical necrosis symptoms. The virus could be transmitted to a wide range of experimental hosts, including a number of solanaceous plants. Purified virus was used to produce specific polyclonal rabbit antibodies and serological tests such as enzyme-linked immunosorbent assay, Western blot analysis, and an immunochromatographic lateral flow assay. Such assays confirmed the wide distribution of this virus in symptomatic field plants in the area of the epidemic. Purified particles contained two genomic RNA molecules of ca. 7 kb (RNA1) and 5 kb (RNA2) estimated length. Analysis of clones from a cDNA library provided 6.5 and 3.0 kb of sequence for RNA1 and RNA2, respectively. Sequence analysis of the encoded replicase showed greatest similarity with members of the Sequiviridae family, and indicated that the virus we isolated is a new virus species, provisionally named Tomato apex necrosis virus.
JnArticleKeywords
Additional keywords:
Solanum lycopersicum
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© 2007 The American Phytopathological Society