September
2007
, Volume
91
, Number
9
Pages
1,083
-
1,088
Authors
Elwin L.
Stewart
,
Xinshun
Qu
,
Barrie E.
Overton
,
Fred E.
Gildow
, and
Nancy G.
Wenner
,
Department of Plant Pathology, The Pennsylvania State University, University Park 16802
; and
Deborah S.
Grove
,
Huck Institute, Wartik Laboratory, The Pennsylvania State University
Affiliations
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RelatedArticle
Accepted for publication 11 April 2007.
Abstract
ABSTRACT
Grapevines infected with Tomato ring spot virus (ToRSV) pose an economic risk for growers in the northeastern United States. This study describes a one-step real-time reverse-transcription polymerase chain reaction (RT-PCR) SYBR Green assay for detecting ToRSV in grapevines. Two newly designed primer pairs based on the ToRSV coat protein gene sequence were evaluated for specificity and optimized for a SYBR Green assay. The primer pair ToRSV1f/1r yielded a 130-bp product with strong primer-dimer products, whereas the primer pair ToRSV2f/2r yielded a 330-bp product with weak primer dimer products. Real-time RT-PCR detected ToRSV in more naturally infected grapevines maintained in the greenhouse than did enzyme-linked immunosorbent assay. The nucleotide sequences of the fragments amplified from grapevine growing in Pennsylvania using real-time PCR were divergent from previously published sequences.
JnArticleKeywords
Additional keywords:
Grape yellow vein virus,
Vitis vinifera
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ArticleCopyright
© 2007 The American Phytopathological Society