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Occurrence of Late Leaf Rust Caused by Pucciniastrum americanum in Red Raspberry (Rubus idaeus) in Buenos Aires, Córdoba, and Entre Ríos, Argentina

In the fall of 2003, severity of late leaf rust in leaves and fruits of red raspberry (Rubus idaeus L.) reached 50% in Buenos Aires (Azul, Baradero, Capilla del Señor, Gral. Rodríguez, Mar del Plata, and Tandil), Córdoba (Villa de Las Rosas), and Entre Ríos (Concordia). The south of Argentina, Río Negro (El Bolsón), and Chubut (El Hoyo, Lago Puelo) remained rust free. The abaxial side of the field infected leaves had pustules filled with masses of yellow spores. Chlorotic areas corresponded in the adaxial side. Urediospores were vacuum harvested from field infected leaves collected in the Tandil area and placed onto a healthy 1-year-old greenhouse-grown plant (cv. Heritage). Spores from a single pustule were increased on plants of the same cultivar. Spores were studied with optic and electronic microscopy. Uredial ostiolar cells were warted, laterally free, and constricted in the middle. The obovoid, echinulate urediospores, from infected leaves averaged 24 × 16 μm (16 to 30 × 11 to 21 μm). Morphological characteristics and spore measurements agreed with those reported for Pucciniastrum americanum (1). Urediniospores were suspended in mineral oil and sprayed onto three raspberry cultivars that were maintained in a darkened mist chamber at 20°C for 48 h and the transferred to a 20°C and 12-h light cycle chamber. Control plants were inoculated with sterile water. There were three replicate plants of each treatment. After 11 days, large sporulating uredia (0.5 mm) were produced on inoculated leaves of cv. Autumn Bliss and smaller uredia (0.1 to 0.3 mm) were produced on cv. Heritage. There were necrotic flecks and the least and smallest uredia were produced on cv. Himbo Queen. No symptoms were present in control plants. To our knowledge, this is the first report of P. americanum causing disease on raspberry in Argentina.

Reference: (1) G. F. Laundon and A. F. Rainbow. Pucciniastrum americanum. No. 210 in: Descriptions of Pathogenic Fungi and Bacteria. CMI, Kew, Surrey, England, 1969.