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Molecular Characterization of Meloidogyne hispanica (Nematoda, Meloidogynidae) by Phylogenetic Analysis of Genes Within the rDNA in Meloidogyne spp.

July 2008 , Volume 92 , Number  7
Pages  1,104 - 1,110

Blanca B. Landa, Instituto de Agricultura Sostenible (IAS), Consejo Superior de Investigaciones Científicas (CSIC), Apdo. 4084, 14080 Córdoba, Spain; Juan E. Palomares Rius, IAS-CSIC; Nicola Vovlas, Istituto per la Protezione delle Piante, Sezione di Bari, Consiglio Nazionale delle Ricerche, (C.N.R.), Via G. Amendola 165/A, 70126 Bari, Italy; Regina M. D. G. Carneiro, Embrapa Recursos Genéticos e Biotecnologia, C.P. 02372, 70849-970, Brasilia, DF, Brazil; Carla M. N. Maleita and Isabel M. de O. Abrantes, Instituto do Mar-CIC, Departamento de Zoologia, Universidade de Coimbra, Coimbra 3004-517, Portugal; and Pablo Castillo, IAS-CSIC



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Accepted for publication 14 March 2008.
ABSTRACT

In the past, the distribution of Meloidogyne hispanica, the Seville root-knot nematode, appeared to be restricted to the southern part of Spain and Prunus spp.; however, its distribution has been confirmed to be worldwide because it occurs in all continents (Europe, Africa, Asia, Australia, and North, Central, and South America). Differentiation of M. hispanica from other Meloidogyne spp., mainly M. arenaria, can be very difficult using morphological and biological traits data. These species are quite similar and can be regularly confused in inaccurate taxonomic comparisons. In this study, species-specific polymerase chain reaction (PCR) and phylogenetic analysis of sequences from three ribosomal (r)DNA regions (18S, internal transcribed spacer [ITS]1-5.8S-ITS2, and D2-D3 of 28S) were used to characterize three M. hispanica isolates from different geographical origins (Brazil, Portugal, and Spain). Molecular analyses showed identical sequences for all three isolates for the three rDNA regions. Maximum parsimony analysis of the three rDNA regions and the species-specific PCR demonstrated and supported the differentiation of M. hispanica from M. incognita, M. javanica, and M. arenaria and from all described root-knot nematode species.


Additional keywords:diagnosis, isozyme phenotypes, morphology

© 2008 The American Phytopathological Society