May
2008
, Volume
92
, Number
5
Pages
808
-
817
Authors
Dallas L. Seifers and
T. J. Martin, Professors, Kansas State University, Agricultural Research Center-Hays, Hays 67601-9228;
Tom L. Harvey, Professor, Department of Entomology, Kansas State University, Manhattan 66506;
John P. Fellers, United States Department of Agriculture--Agricultural Research Service--PSERU, Department of Plant Pathology, Manhattan, KS 66506;
James P. Stack and
Marietta Ryba-White, Department of Plant Pathology, Kansas State University, Manhattan;
Steve Haber, Cereal Research Centre, Agriculture & Agri-Food Canada, Winnipeg, Manitoba, Canada; and
Oleg Krokhin,
Victor Spicer,
Nicole Lovat,
Andrej Yamchuk, and
Kenneth G. Standing, Department of Physics and Astronomy, University of Manitoba, Winnipeg, Manitoba, Canada
Affiliations
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RelatedArticle
Accepted for publication 15 January 2008.
Abstract
ABSTRACT
In 2006, a mechanically-transmissible and previously uncharacterized virus was isolated in Kansas from wheat plants with mosaic symptoms. The physiochemical properties of the virus were examined by purification on cesium chloride density gradients, electron microscopy, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), sequencing of the nucleotides and amino acids of the coat protein, and immunological reactivity. Purified preparations contained flexuous, rod-shaped particles that resembled potyviruses. The coat protein was estimated from SDS-PAGE to have a mass of approximately 35 kDa. Its amino acid sequence, as deduced from DNA sequencing of cloned, reverse-transcribed viral RNA and separately determined by time-of-flight mass spectrometry, was most closely related (49% similarity) to Sugarcane streak mosaic virus, a member of the Tritimovirus genus of the family Potyviridae. The virus gave strong positive reactions during enzyme-linked immunosorbent assays using polyclonal antibodies raised against purified preparations of the cognate virus but gave consistent negative reactions against antibodies to Wheat streak mosaic virus (WSMV), other wheat potyviruses, and the High Plains virus. When the virus was inoculated on the WSMV-resistant wheat cv. RonL, systemic symptoms appeared and plant growth was diminished significantly in contrast with WSMV-inoculated RonL. Taken together, the data support consideration of this virus as a new potyvirus, and the name Triticum mosaic virus (TriMV) is proposed.
JnArticleKeywords
Additional keywords:temperature-sensitive resistance
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© 2008 The American Phytopathological Society