October
2008
, Volume
92
, Number
10
Pages
1,387
-
1,393
Authors
Fulya
Baysal-Gurel
,
Melanie L. Lewis
Ivey
, and
Anne
Dorrance
,
Department of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster
;
Douglas
Luster
and
Reid
Frederick
,
USDA ARS Foreign Diseases and Weed Science Research Unit, Ft. Detrick, MD
;
Jill
Czarnecki
,
Naval Medical Research Center, Biological Defense Research Directorate, Silver Spring, MD
;
Michael
Boehm
,
Department of Plant Pathology, The Ohio State University, Columbus
; and
Sally A.
Miller
,
Department of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster
Affiliations
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RelatedArticle
Accepted for publication 6 June 2008.
Abstract
ABSTRACT
An indirect immunofluorescence spore assay (IFSA) was developed to detect urediniospores of Phakopsora pachyrhizi, utilizing rabbit polyclonal antisera produced in response to intact nongerminated (SBR1A) or germinated (SBR2) urediniospores of P. pachyrhizi. Both antisera were specific to Phakopsora spp. and did not react with other common soybean pathogens or healthy soybean leaf tissue in enzyme-linked immunosorbent assay (ELISA). SBR1A and SBR2 bound to P. pachyrhizi and P. meibomiae urediniospores were detected with goat anti-rabbit Alexa Fluor 488-tagged antiserum using a Leica DM IRB epifluorescent microscope with an I3 blue filter (excitation 450 to 490 nm, emission 515 nm). The assay was performed on standard glass microscope slides; double-sided tape was superior to a thin coating of petroleum jelly both in retaining spores and in immunofluorescence. The IFSA was used to confirm the identity of P. pachyrhizi urediniospores captured on glass slides from passive air samplers from Georgia, Kentucky, and Ohio during 2006.
JnArticleKeywords
Additional keywords:
field trap,
soybean rust
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ArticleCopyright
© 2008 The American Phytopathological Society