Authors
T. Mizutani, Department of International Agricultural Development, Graduate School of Agriculture, Tokyo University of Agriculture, 1-1-1, Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan; B. S. Daryono, Faculty of Biology, Gadjah Mada University, Yogyakarta 55281, Indonesia; M. Ikegami, NODAI Research Institute, Tokyo University of Agriculture, 1-1-1, Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan; and K. T. Natsuaki, Department of International Agricultural Development, Tokyo University of Agriculture, 1-1-1, Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan
Cucumber (Cucumis sativus L.) is an important vegetable in Indonesia. Cucumber plants showing yellowy green mosaic symptoms on leaves were observed in Klaten, Central Java, Indonesia in August 2008. Total DNAs were extracted from symptomatic leaves, and the putative viral genomes were amplified by PCR with the Deng A and B primers (2). The PCR-amplified viral genomic DNA was sequenced. The remaining part of DNA-A was amplified with two primers sets (ToLCNDV-A1F 5′-ACCAACAGGCCGATGAACA-3′ and ToLCNDV-A1R 5′-TTCCCACTATCTTCCTGTGCA-3′; ToLCNDV-A2F 5′-TCGAGTGTGATRAAGAYTGCA-3′ and ToLCNDV-A2R 5′-ACTAACTAAGCATTGCAGCGTC-3′ [R = A and G, Y = C and T]) and sequenced. The remaining part of DNA-B was amplified with two primers sets (ToLCNDV-B1F 5′-ARGAGTTYMCRYYTGTGGA-3′ and ToLCNDV-B1R 5′-TKCWGTYGGTCATGTCGT-3′; ToLCNDV-B2F 5′-TCYGTCAATCKCATGTCGYGT-3′ and ToLCNDV-B2R 5′-CCTTACGCGTATAYTGTYTRGA-3′ [K = G and T, M = A and C, W = A and T]) and sequenced. Full-length DNA-A (2,739 nt; GenBank Accession No. AB613825) and DNA-B (2,690 nt; GenBank Accession No. AB613826) sequences of a bipartite Tomato leaf curl New Delhi virus (ToLCNDV) from Central Java were obtained and they were most similar to the corresponding sequences of both DNA-A and DNA-B of ToLCNDV-[cucumber:Thailand] (DNA-A, GenBank Accession No. AB330079; DNA-B, GenBank Accession No. AB330080) at 95.5 and 91.0% nucleotide identities, respectively. On the basis of high nucleotide sequence identity with ToLCNDV-[cucumber:Thailand] and the demarcation criteria in species identification (3), the virus isolate from the diseased cucumber in Central Java is considered as a variant of ToLCNDV and was accordingly named ToLCNDV-Indonesia[Indonesia:Java:Cucumber:2008] (ToLCNDV-ID[ID:Jav:Cuc:08]). Although the importance of begomovirus diseases on chili pepper (Solanaceae) is currently highly noticed in Indonesia (1), ToLCNDV was newly isolated from cucumber (Cucurbitaceae) in this study. Therefore, farmers in Indonesia should pay more attention to controlling begomovirus vectors, white flies, on Cucurbitaceae. To our knowledge, this is the first report of the natural occurrence of ToLCNDV in Indonesia.
References: (1) P. J. D. Barro et al. Biol. Invas.10:411, 2008. (2) D. Deng et al. Ann. Appl. Biol. 125:327, 1994. (3) C. M. Fauquet et al. Arch. Virol. 153:783, 2008.
